Dermatol. praxi. 2026;20(1):15-19 | DOI: 10.36290/der.2026.002

Direct immunofluorescence in dermatology and dermatopathology

MUDr. Miroslav Důra, Ph.D.1, 2
1 Dermatovenerologická klinika, 1. LF UK a VFN, Praha
2 Bioptická laboratoř, s. r. o., Plzeň

Examination of the presence of tissue-bound antibodies, complement components, or fibrinogen by direct immunofluorescence plays an indispensable role in dermato­logy and dermatopathology. For several diagnoses, particularly autoimmune bullous dermatoses, it even constitutes a diagnostic criterion. The following text focuses on the principles of this examination, its indications, benefits, and limitations. The immunofluorescence patterns of individual dermatoses are discussed. Practical information regarding proper specimen collection and transport is also included.

Keywords: direct immunofluorescence, dermatopathology, bullous dermatoses, pemphigus, pemphigoid, vasculitis.

Accepted: January 12, 2026; Published: April 8, 2026  Show citation

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Důra M. Direct immunofluorescence in dermatology and dermatopathology. Dermatol. praxi. 2026;20(1):15-19. doi: 10.36290/der.2026.002.
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